Transgenic control of insect pests in maize reduces mycotoxin concentrations in grain
Gary P. Munkvold, and R.L. Hellmich
Iowa State University Depts. of Plant Pathology and Entomology and USDA-ARS
The safety of food and feed products for consumption should be the primary consideration in regard to regulation of agricultural and food production industries. In terms of human food safety, foodborne microorganisms pose the greatest threat, followed by mycotoxins produced by fungi in food products. For the safety of livestock feeds, mycotoxins are recognized as the greatest hazard. Mycotoxin-producing fungi include species of Aspergillus, Fusarium, Penicillium, and others. Maize kernels grown throughout the world are commonly infected by mycotoxin-producing fungi in the genus Fusarium. Worldwide, the most common Fusarium mycotoxins in maize are the fumonisins. This group of toxins can cause fatal diseases in livestock and are carcinogenic. Conventional breeding methods have not been successful in developing maize hybrids with high levels of resistance to these fungi because of the lack of major resistance genes in maize. Infection of maize by Fusarium species is greatly influenced by insect pests such as the European corn borer. Insect larvae carry spores of the fungi to the maize kernels and deposit them there. Furthermore, injuries to the kernel tissue caused by insects are very vulnerable sites for fungal infection. Therefore, there is a correlation between insect damage and fumonisin concentrations in maize kernels. We therefore investigated the potential for reducing fumonisin concentrations in maize kernels through insect control. Highly effective insect resistance offered by transgenic Bt hybrids expressing CryIA(b) or Cry9C in kernel tissue successfully prevented the accumulation of significant fumonsin concentrations. Near-isogenic, conventional hybrids often had fumonisin concentrations 10 times higher than those in the kernels of these Bt hybrids. Partial insect control by synthetic insecticides, foliar applications of Bacillus thuringiensis, or Bt hybrids lacking kernel expression of Cry proteins, resulted in some less dramatic reductions in fumonisin concentrations. Similar results have been reported from other states in the U.S. and from Italy. These results indicate that the safety of maize grain for consumption by humans and livestock can be enhanced through the use of transgenic insect control. Bt hybrids are currently the most effective management tool available for reducing fumonisin concentrations in maize grain.

Ear samples from a 1997 field trial.
Non-Bt hybrid is heavily damaged by insect feeding and Fusarium ear rot, but the near-isogenic Bt hybrid has little or no damage.

A summary of this work is available at : http://www.scisoc.org/feature/BtCorn/Top.html
Related information can be found at: http://www.scisoc.org/feature/BtCorn/0422-04F.pdf